CV

EDUCATION

2010

PhD, Dept of Molecular Genetics, University of Toronto, Ontario, Canada

Thesis: Analysis of the Nucleoprotein Complexes Essential for Plasmid Partition

2003

Honors Bachelor of Science – with High Distinction

Dept of Molecular Genetics, University of Toronto, Ontario, Canada

 

RESEARCH EXPERIENCE

2014-Present

Research Fellow, National Institutes of Health (NIH)

Advisor – Dr. Kiyoshi Mizuuchi

  • Managing lab and supervising four research projects
  • Developing cell-free imaging techniques

 

2010-2014

Postdoctoral Fellow, National Institutes of Health

Advisor – Dr. Kiyoshi Mizuuchi

  • Developed cell-free imaging to study self-organizing systems
  • Reconstituted bacterial DNA segregation & cell division positioning

 

2003-2010

Graduate Student, Dept of Molecular Genetics, University of Toronto

Advisor – Dr. Barbara Funnell

  • Developed expertise in molecular biology, biochemistry and genetics
  • Identified and characterized complexes required for bacterial mitosis

 

2001-2003

Undergrad Research, Dept of Molecular Genetics, University of Toronto

Advisor – Dr. Barbara Funnell

  • Studied the kinetochore protein required for bacterial mitosis

 

FELLOWSHIPS

2010-Present

Nancy Nossal Postdoctoral Fellowship, NIH

2004-2009

Open Fellowship, University of Toronto

2007

L.W. Macpherson Microbiology Fellowship, University of Toronto

2005

Eric Hani Microbiology Fellowship, University of Toronto

 

HONORS AND AWARDS

2014

Cozzarelli Prize, Division of Biological Sciences, National Academy of Sciences

2013-2014

Earl Stadtman Investigator Semifinalist, NIH

2013-2014

Fellows Award for Research Excellence, NIH

2012

Best Oral Presentation – NIDDK Postdoctoral Conference, NIH

2010

Barbara Vivash Award – Best PhD Thesis, University of Toronto

2009

Molecular Genetics Poster Competition (2nd), University of Toronto

2006

Molecular Genetics Poster Competition (1st), University of Toronto

 

TEACHING EXPERIENCE AND PROFESSIONAL TRAINING

2015-Present

Mentor, Kiyoshi Mizuuchi lab, NIH

Currently supervising four research projects in a variety of disciplines

2014                           

Attendee & Speaker, Synthetic Biology Workshop, University of Toronto

A one week course focused on the current state of synthetic biology

2012-2013

Lecturer, Foundation for Advanced Education in the Sciences (FAES), NIH

Molecular Biology & Genetics – Introductory course

2012

Mentor Training Certificate, Intramural Training & Education, NIH

2008-2009

Teaching Fundamentals Certificate, Training Program, University of Toronto

2005-2009

Laboratory Demonstrator &Teaching Assistant, University of Toronto

Principles of Genetic Analysis under the coordination of Dr. B.E. Funnell

2001-2002

Science OutreachTM Instructor, Faculty of Engineering, University of Toronto

 

LEADERSHIP, COMMUNITY & SERVICE

2013-Present

Ad-Hoc Reviewer, Applied and Environmental Microbiology

2013-Present

Ad-Hoc Reviewer, Journal of Bacteriology

2011-2013

Judge, Montgomery County Science Fairs, MD, USA

2004-2005

Member, Varsity Blues Men’s Volleyball Team, University of Toronto

2003-2004

1st Year Representative, Grad Student Association, University of Toronto

 

SELECTED INVITED TALKS

2015 – Organizing the Cell with Protein Gradients.

American Society for Cell Biology, Annual Meeting. San Diego, CA, USA

 

2015 – Bacterial DNA Segregation: No spindle required.

Nucleic Acids, Gordon Research Conference. Biddeford, ME, USA

 

2015 – Spatial Regulators of Bacterial Cell Division form a Standing Wave Oscillator.

Prokaryotic Cell Biology, American Society for Microbiology. Washington, DC, USA

 

2014 – Intracellular transport and positioning by dynamic protein gradients on biological surfaces.

Earl Stadtman Symposium. NIH, Bethesda, MD, USA

 

2014 – A propagating ATPase gradient drives transport of surface-confined cellular cargo.

American Physical Society, Annual Meeting. Denver, CO, USA

 

2013 –  Surfing the nucleoid: A key role for spatial confinement in ParA-mediated transport.

Chromosome Dynamics, Gordon Research Conference. Barga, Italy

American Society for Microbiology, Annual Meeting. Denver, CO, USA

The Bauer Forum. Harvard University

 

2008 –  Identifying a Role for the Host Nucleoid in Plasmid Partition.

Molecular Mechanisms Meeting. University of Western Ontario, Canada

 

POSTER ABSTRACTS

2015 – The bacterial Min system self-organizes into a standing wave oscillator on a flat bilayer in vitro.    

American Society for Cell Biology, Annual Meeting. San Diego, CA, USA

Biophysical Society, Annual Meeting. Baltimore, MD, USA

Boston Bacterial Meeting. Boston, MA, USA

 

2015 –  Directed and persistent movement arises from mechanochemistry of the ParA/ParB system            

Chromosome Dynamics, Gordon Research Conference. Waterville Valley, NH, USA      

 

2014 –  A propagating ATPase gradient drives transport of surface-confined cellular cargo.          

Biophysical Society, Annual Meeting. San Francisco, CA, USA

 

2013 –  Surfing the nucleoid: A key role for spatial confinement in ParA-mediated transport.

Chromosome Dynamics, Gordon Research Conference. Barga, Italy

 

2012 –  Transport of DNA via self-organized protein patterning on a “nucleoid” surface.

Chromosome Dynamics Workshop. Woods Hole, MA, USA

 

2012 –  Visualizing bacterial DNA segregation in a cell-free   system.          

Biophysical Society Meeting, Annual Meeting. San Diego, CA, USA

 

2009 – A role for the host chromosome in plasmid segregation and movement.

Chromosome Dynamics, Gordon Research Conference. Barga, Italy

 

2007 –  Partition Complex Assembly Involves Several Modes of Protein-DNA Recognition.

International Symposium on Plasmid Biology. Lake Tahoe, CA, USA

PUBLICATIONS

Vecchiarelli AG, Li M, Mizuuchi M, Hwang LC, Seol Y, Neuman KC & Mizuuchi K (2016). Membrane-bound MinDE complex acts as a toggle switch that drives Min oscillation coupled to cytoplasmic depletion of MinDPNAS

 

Longhua Hu, Vecchiarelli AG, Mizuuchi K, Neuman KC & Liu J (2015). Directed and persistent movement arises from mechanochemistry of the ParA/ParB system. PNAS, E7055

 

Vecchiarelli AG*, Taylor JA & Mizuuchi K. ParAB motility reconstitution (2015). Building a Cell from its Component Parts. Methods in Cell Biology 128, Chapter 13

* Corresponding author

 

Vecchiarelli AG, Seol Y, Neuman KC & Mizuuchi K (2015). A moving ParA gradient on the nucleoid directs subcellular cargo transport via a chemophoresis force. BioArchitecture 4, 154

 

Vecchiarelli AG, Li M, Mizuuchi M & Mizuuchi K (2014). Differential affinities of MinD and MinE to anionic phospholipid influence Min patterning dynamics in vitro. Mol Microbiol 93, 453

 

Vecchiarelli AG, Neuman KC & Mizuuchi K (2014). A propagating ATPase gradient drive transport of surface-confined cellular cargoPNAS 111, 4880

 

Vecchiarelli AG, Havey JC, Ing L, Wong E, Waples W & Funnell BE (2013). Dissection of the ATPase active site of P1 ParA reveals multiple active forms essential for plasmid partition. J Biol Chem 288, 17823

 

Vecchiarelli AG, Hwang LC & Mizuuchi K (2013). Cell-free study of F plasmid partition provides evidence for cargo transport by a diffusion-ratchet mechanism. PNAS 110, E1390

 

Hwang LC*, Vecchiarelli AG*, Han YW, Mizuuchi M, Harada Y, Funnell BE & Mizuuchi K (2013). ParA-mediated plasmid partition driven by protein pattern self-organization. EMBO J 32, 1238

* Equal Contribution

 

Vecchiarelli AG & Funnell BE (2013). Probing the N-terminus of ParB using cysteine-scanning mutagenesis & thiol modification. Plasmid 70, 86

 

Vecchiarelli AG, Mizuuchi K & Funnell BE (2012). Surfing biological surfaces: exploiting the nucleoid for partition and transport in bacteria. Mol Microbiol 86, 513

 

Havey JC, Vecchiarelli AG & Funnel BE (2012). ATP-regulated interactions between P1 ParA, ParB & non-specific DNA that are stabilized by the plasmid partition site. Nucl Acids Res 40, 801

 

Vecchiarelli AG, Han YW, Tan X, Mizuuchi M, Ghirlando R, Biertümpfel C, Funnell BE & Mizuuchi K (2010). ATP control of dynamic P1 ParA-DNA interactions: a key role for the nucleoid in plasmid partition. Mol Microbiol 78, 78

 

Vecchiarelli AG, Schumacher MA & Funnell BE (2007). P1 partition complex assembly involves several modes of protein-DNA recognition. J Biol Chem 282, 10944

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